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What Is Urolithin A? Mitophagy, Your Microbiome, and What Human Trials Measured

Published 2026-09-16 · Last reviewed 2026-09-16 · By Andy Palenzuela, Founder

Urolithin A is the rare longevity ingredient with an unusual story: it is not in any food, your body cannot make it on its own, and whether you produce it at all depends on which bacteria happen to live in your gut. This article explains what urolithin A is, how it gets made (or does not), what mitophagy means in plain English, what the two human trials measured at 500 and 1,000 mg, and how to read a urolithin A label. It is written by the founder of Age Revive, who spent 14+ years inside regulated nutrition supply chains before formulating CELLUBIOME around 500 mg of urolithin A.

Key facts

  • Urolithin A: is a small molecule produced by certain gut bacteria from ellagitannins — polyphenols in pomegranate, raspberries, strawberries, and walnuts. It is not found in food itself.
  • Most people make little of it: Production depends on carrying the right bacterial species; the amount produced varies widely from person to person and can be close to zero.
  • Mitophagy: is the process cells use to identify worn-out mitochondria and recycle them. Urolithin A is studied for its role in supporting this pathway.
  • Human trials: 250, 500, and 1,000 mg/day for 28 days was well tolerated in adults 61–85, with a mitochondrial gene-expression signature at 500 and 1,000 mg (Andreux 2019); 500 and 1,000 mg/day for four months in middle-aged adults reported changes in muscle-strength, exercise-performance, and mitochondrial-health biomarkers versus placebo (Singh 2022).
  • Doses studied: 250–1,000 mg/day. CELLUBIOME provides 500 mg/day at ≥99% purity, paired with 500 mg enteric-coated tributyrin.
  • Neither trial tested a finished supplement: They tested urolithin A as a single compound. CELLUBIOME was not in either.

What urolithin A is

Urolithin A is a dibenzopyranone — a small, two-ring molecule that belongs to a family of compounds called urolithins. It is a metabolite: something produced by the transformation of another molecule, not a nutrient that exists in the diet as-is.

The chain of custody runs like this:

  1. You eat ellagitannins. These are large polyphenols concentrated in pomegranate, certain berries, and walnuts.
  2. Your gut converts them to ellagic acid. This step is reliable and happens in most people.
  3. Specific gut bacteria convert ellagic acid to urolithins. This is the step that varies. Only certain bacterial species carry the enzymes to do it, and urolithin A is one of several possible end products.
  4. Urolithin A is absorbed and circulates, mostly as conjugated forms, before being cleared.

Steps one and two are reliable. Step three is the bottleneck, and it is why urolithin A exists as a supplement at all.

Why most people don't make enough of it

Research suggests most people's microbiomes do not convert ellagic acid to urolithin A efficiently. The bacteria responsible are not universal, and even among people who carry them, output varies widely. Researchers describe people by urolithin "metabotype" — roughly, those who produce urolithin A, those who produce other urolithins instead, and those who produce essentially none.

The practical consequence is blunt: eating pomegranate does not guarantee urolithin A. Two people can drink the same juice and end up with very different blood levels, and one of them may end up with nothing measurable. A direct urolithin A supplement bypasses step three entirely — it supplies the end product whether or not your bacteria can make it.

What mitophagy is, in plain English

Mitochondria are the parts of the cell that turn food-derived fuel into usable energy. They are also disposable. Individual mitochondria wear out, accumulate damage, and become less efficient, and a cell that keeps too many worn-out ones runs worse than a cell that recycles them.

Mitophagy is the recycling program. A damaged mitochondrion is tagged by the cell, wrapped in a membrane, delivered to the cell's digestive compartment, and broken down into components that can be reused. Clearing old mitochondria makes room for the cell to produce fresh ones. The word combines mito (mitochondria) and phagy (eating) — the cell eats its own worn-out parts on purpose.

Urolithin A is studied for its role in supporting mitophagy. In the human research, that support has been measured as changes in mitochondrial biomarkers — gene-expression signatures in muscle tissue and metabolite patterns in blood — rather than as a direct count of recycled mitochondria. "Supports mitochondrial renewal through the mitophagy pathway" is the structure/function description; it is not a claim that the process is switched on like a light.

What the two human trials measured

Andreux 2019 — first-in-human safety and biomarkers

Published in Nature Metabolism, this was a randomized, double-blind, placebo-controlled trial in 60 sedentary older adults aged 61 to 85. It ran in two phases: a single-dose phase, then a 28-day phase in which participants took 250, 500, or 1,000 mg of urolithin A daily, or placebo.

The primary endpoint was safety and tolerability. Urolithin A was reported as safe and well tolerated at all doses. Secondary and exploratory measures included plasma acylcarnitines, which decreased, and skeletal-muscle gene expression, where the authors reported a mitochondrial gene-expression signature at the 500 mg and 1,000 mg doses. The study did not report improvements in physical performance over the four weeks.

What it established: urolithin A is well tolerated up to 1,000 mg/day for 28 days in this population, and the 500 and 1,000 mg doses were associated with a mitochondrial biomarker signature. What it did not: any functional outcome, and nothing beyond four weeks.

Singh 2022 — four months at 500 and 1,000 mg

Published in Cell Reports Medicine, this was a randomized, placebo-controlled trial in middle-aged adults who took 500 mg or 1,000 mg of urolithin A daily for four months. The authors reported changes in muscle-strength and exercise-performance measures and in mitochondrial-health biomarkers versus placebo.

What it established: a longer exposure (four months) at the same two doses, with biomarker and performance-measure findings reported at the study level. What it did not: anything about a finished supplement, and its trial averages are not a promise of individual results. Age Revive does not make strength or performance claims for CELLUBIOME on the basis of this study.

Citation hygiene note for readers who look these up: Andreux et al. is the 2019 Nature Metabolism paper. Singh et al. is the 2022 Cell Reports Medicine paper. They are frequently confused online.

What dose was studied — and where does 500 mg sit?

TrialDoseDurationPopulationPrimary finding
Andreux 2019250 / 500 / 1,000 mg/day28 days60 sedentary adults, 61–85Safe and well tolerated; mitochondrial gene-expression signature at 500 and 1,000 mg
Singh 2022500 / 1,000 mg/day4 monthsMiddle-aged adultsChanges in muscle-strength, exercise-performance, and mitochondrial-health biomarkers vs placebo

500 mg is the dose that appears in both trials and the lower of the two that produced a biomarker signature in Andreux 2019. 250 mg was tested in Andreux only and did not produce the same signature.

CELLUBIOME provides 500 mg of urolithin A per daily serving (2 enteric-coated capsules), at ≥99% purity. The current lot certificate, published on our quality page, shows urolithin A tested at 548.15 mg per serving against a label claim of not less than 500 mg. The dose is aligned with the published human research. It is not "dose-matched" — the trials tested the compound alone, not CELLUBIOME, and not alongside tributyrin.

Why pair urolithin A with tributyrin

Urolithin A is a gut-derived molecule that works downstream, in the mitochondria. Left on its own in a formula, it says nothing about the gut it came from — specifically the colon lining, whose cells run on a short-chain fatty acid called butyrate. CELLUBIOME pairs 500 mg urolithin A with 500 mg tributyrin (a butyrate triglyceride designed to survive stomach acid) so that one serving supports both the gut barrier and mitochondrial renewal. Not just gut. Mitochondria too. The reasoning is covered in Urolithin A + Tributyrin: Gut Barrier and Mitochondrial Support, and the delivery choice in Tributyrin vs Sodium Butyrate.

Is urolithin A safe?

In the human trials above, urolithin A was well tolerated at up to 1,000 mg/day over 28 days (Andreux 2019) and at 500 and 1,000 mg/day over four months (Singh 2022). Those are study populations of healthy adults; they are not a clearance for everyone. If you are pregnant, breastfeeding, managing a condition, or taking medication, speak with your clinician before starting any supplement.

What the research does not show

  • Urolithin A has not been shown to extend human lifespan. Lifespan findings in this field come from animal and laboratory models and do not transfer to people.
  • Urolithin A has not been shown to "boost energy" or "reverse aging." The human data are tolerability and biomarker data, plus performance measures at the study level in one trial.
  • No trial has tested CELLUBIOME. The trials tested urolithin A alone.
  • Supporting mitophagy is not a treatment for any muscular, metabolic, or age-related condition.
  • Pomegranate is not a substitute for a measured dose, and a supplement is not a substitute for a diet that contains polyphenols and fiber.

How to read a urolithin A label

  1. Dose per serving. 500–1,000 mg/day is the range that produced biomarker findings. 100 or 250 mg on a label is a fraction of that.
  2. Purity. Look for a stated purity (CELLUBIOME's is ≥99%). "Pomegranate extract standardized for ellagic acid" is not urolithin A — it is the precursor your bacteria may or may not convert.
  3. A published lot certificate with a lab name, an accreditation, and a tested value for urolithin A specifically.
  4. What it is paired with. Urolithin A works downstream of the gut. Ask what the formula does for the gut wall itself.
  5. Whether it is a probiotic in disguise. Urolithin A is a metabolite, not a live culture. If a label lists CFU counts next to it, they are doing different jobs — covered in Urolithin A vs Probiotics.

Frequently asked questions

What does urolithin A do? Urolithin A is studied for its role in supporting mitophagy — the process by which cells recycle worn-out mitochondria. In human trials at 500–1,000 mg/day it was well tolerated and associated with changes in mitochondrial biomarkers. It is a gut-derived metabolite, not a probiotic.

Can I get urolithin A from pomegranate? Only if your gut bacteria convert it. Pomegranate contains ellagitannins, the raw material; specific bacteria turn them into urolithin A, and research suggests most people's microbiomes do not do this efficiently. Supplemental urolithin A supplies the end product directly.

What is the right urolithin A dose? Human trials used 250, 500, and 1,000 mg per day. The 500 mg and 1,000 mg doses were associated with mitochondrial biomarker changes; 250 mg was not, in the one trial that tested it. CELLUBIOME provides 500 mg per daily serving. Dose decisions belong with your clinician.

Is urolithin A safe to take every day? In published human trials, daily urolithin A at up to 1,000 mg was well tolerated over 28 days and, at 500–1,000 mg, over four months. Those trials were in healthy adults; consult your clinician if you are pregnant, managing a condition, or on medication.

Sources

  1. Andreux PA, Blanco-Bose W, Ryu D, et al. The mitophagy activator urolithin A is safe and induces a molecular signature of improved mitochondrial and cellular health in humans. Nature Metabolism. 2019;1:595–603. doi:10.1038/s42255-019-0073-4. PMID 32694802.
  2. Singh A, D'Amico D, Andreux PA, et al. Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults. Cell Reports Medicine. 2022;3(5):100633. doi:10.1016/j.xcrm.2022.100633. PMID 35584623.
  3. Age Revive. CELLUBIOME Lot 150126 third-party certificate of analysis

Related reading

  • Urolithin A + Tributyrin: Gut Barrier and Mitochondrial Support — Urolithin A has a human study behind it; tributyrin is a butyrate delivery choice. What Andreux 2019 measured and why CELLUBIOME is not a probiotic.
  • How to Choose a Urolithin A Supplement: The Six-Check Label Test — Six checks that separate real urolithin A supplements from precursors and trace-dose products, from studied dose and purity to price.
  • Urolithin A vs Probiotics: Two Different Jobs in the Gut — Probiotics address bacteria in the gut; urolithin A is a metabolite studied for its role in mitochondria. They address two different problems.
  • Tributyrin vs Sodium Butyrate: Why Delivery Decides What Reaches Your Gut — Sodium butyrate and tributyrin deliver butyrate differently. This comparison explains their chemistry, delivery, and what the research actually measured.

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About the author

Andy Palenzuela is the founder of Age Revive and has 14+ years of experience in regulated nutrition supply chains, including manufacturing oversight, ingredient sourcing, and quality assurance for consumer health products. Age Revive formulas are developed based on published peer-reviewed research and manufactured at cGMP-certified facilities. LinkedIn

These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. The information in this article is for educational purposes only and is not intended as medical advice. Consult your healthcare provider before starting any supplement regimen.